This is a single molecule localisation microscopy method which combines DNA oligo-nucleotide hybridisation with TIRF microscopy to achieve 5-15 nm in optical resolution for mapping protein, DNA and RNA targets. qPAINT is a target ‘counting’ algorithm which exploits the idea that DNA-PAINT localisation frequency, by the binding kinetics of ssDNA, is related to the underlying DNA target density. We have exploited this for ‘counting’ protein targets within intracellular nanodomains (paper in press with Cell Reports).

Read more about DNA-PAINT: Jungmann et al 2014, Auer et al 2017